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The citrus flavonone hesperetin attenuates the nuclear translocation of aryl hydrocarbon receptor.
- Source :
-
Comparative Biochemistry & Physiology Part C: Toxicology & Pharmacology . Aug2018, Vol. 210, p57-64. 8p. - Publication Year :
- 2018
-
Abstract
- The environmental polycyclic aromatic hydrocarbons (PAH) and dioxins are carcinogens and their adverse effects have been largely attributed to the activation of AhR. Hesperetin is a flavonone found abundantly in citrus fruits and has been shown to be a biologically active agent. In the present study, the effect of hesperetin on the nuclear translocation of AhR and the downstream gene expression was investigated in MCF-7 cells. Confocal microscopy indicated that 7, 12-dimethylbenz[α]anthracene (DMBA) or 2,3,7,8-tetrachlorodibenzo- p- dioxin (TCDD) -induced nuclear translocation of AhR was deterred by hesperetin treatment. The reduced nuclear translocation could also be observed in Western analysis. Reporter-gene assay further illustrated that the induced XRE transactivation was weakened by the treatment of hesperetin. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay demonstrated that the gene expressions of CYP1A1 , 1A2 , and 1B1 followed the same pattern of AhR translocation. These results suggested that hesperetin counteracted AhR transactivation and suppressed the downstream gene expression. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15320456
- Volume :
- 210
- Database :
- Academic Search Index
- Journal :
- Comparative Biochemistry & Physiology Part C: Toxicology & Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 129754035
- Full Text :
- https://doi.org/10.1016/j.cbpc.2018.05.007