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Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface.
- Source :
-
Science . 5/4/2018, Vol. 360 Issue 6388, p552-558. 7p. 4 Diagrams. - Publication Year :
- 2018
-
Abstract
- Kinetochores connect mitotic-spindle microtubules with chromosomes, allowing microtubule depolymerization to pull chromosomes apart during anaphase while resisting detachment as the microtubule shortens. The heterodecameric DASH/Dam1 complex (DASH/Dam1c), an essential component of yeast kinetochores, assembles into a microtubule-encircling ring. The ring associates with rodlike Ndc80 complexes to organize the kinetochore-microtubule interface. We report the cryo–electron microscopy structure (at ~4.5-angstrom resolution) of a DASH/Dam1c ring and a molecular model of its ordered components, validated by evolutionary direct-coupling analysis. Integrating this structure with that of the Ndc80 complex and with published interaction data yields a molecular picture of kinetochore-microtubule attachment, including how flexible, C-terminal extensions of DASH/Dam1c subunits project and contact widely separated sites on the Ndc80 complex rod and how phosphorylation at previously identified sites might regulate kinetochore assembly. [ABSTRACT FROM AUTHOR]
- Subjects :
- *KINETOCHORE
*YEAST fungi
*ELECTRON microscopes
*MOLECULAR models
*PHOSPHORYLATION
Subjects
Details
- Language :
- English
- ISSN :
- 00368075
- Volume :
- 360
- Issue :
- 6388
- Database :
- Academic Search Index
- Journal :
- Science
- Publication Type :
- Academic Journal
- Accession number :
- 129474233
- Full Text :
- https://doi.org/10.1126/science.aar6436