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Distinct Roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/mIRNA Silencing Pathways.
- Source :
-
Cell . 4/2/2004, Vol. 117 Issue 1, p69-81. 13p. - Publication Year :
- 2004
-
Abstract
- The RNase III enzyme Dicer processes RNA into si-RNAs and miRNAs, which direct a RNA-induced silencing complex (RISC) to cleave mRNA or block its translation (RNAi). We have characterized mutations in the Drosophila dicer-1 and dicer-2 genes. Mutation in dicer-1 blocks processing of miRNA precursors, Whereas dicer-2 mutants are defective for processing siRNA precursors. It has been recently found that Drosophila Dicer-1 and Dicer-2 are also components of siRNA-dependent RISC (siRISC). We find that Dicer-1 and Dicer-2 are required for siRNA-directed mRNA cleavage, though the RNase III activity of Dicer-2 is not required. Dicer-1 and Dicer-2 facilitate distinct steps in the assembly of siRISC. However, Dicer-I but not Dicer-2 is essential for miRISC-directed translation repression. Thus, siRlSCs and miRISCs are different with respect to Dicers in Drosophila. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ENZYMES
*CELLS
*RNA
*DROSOPHILA
*DICEROS
*DROSOPHILIDAE
Subjects
Details
- Language :
- English
- ISSN :
- 00928674
- Volume :
- 117
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- 12941140
- Full Text :
- https://doi.org/10.1016/S0092-8674(04)00261-2