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Characterization of highly branched dextran produced by Leuconostoc citreum B-2 from pineapple fermented product.
- Source :
-
International Journal of Biological Macromolecules . Jul2018, Vol. 113, p45-50. 6p. - Publication Year :
- 2018
-
Abstract
- A strain Leuconostoc citreum B-2 was isolated from homemade fermentation product of pineapple and its polysaccharide yield was 28.3 g/L after cultivating the strain in Man-Rogosa-Sharpe (MRS) medium with 75 g/L sucrose. The exopolysaccharide (EPS) was characterized by gas chromatography (GC), Fourier-transform infrared (FT-IR) spectra, high-performance size-exclusion chromatography (HPSEC), nuclear magnetic resonance (NMR) spectroscopy and scanning electron microscope (SEM) analysis in present study. The monosaccharide composition of EPS was glucose and molecular weight was 3.77 × 10 6 Da. FT-IR and NMR spectra revealed that the B-2 EPS was composed of 75% α-(1 → 6) linked d -glucopyranose units existing in the main chain with 19% α-(1 → 3) branching and only a few α-(1 → 2) branching. The SEM of the dried EPS appeared irregular sheets with glittering surface and compact structure. Water solubility index and water holding capacity of B-2 EPS were 80% and 450%, respectively. All the mentioned characteristics suggested that the EPS has a potential application in the food, cosmetic and pharmaceuticals industry. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 113
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 129273155
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2018.02.119