Back to Search
Start Over
Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI–MS/MS.
- Source :
-
Journal of Pharmaceutical & Biomedical Analysis . May2018, Vol. 154, p31-39. 9p. - Publication Year :
- 2018
-
Abstract
- The human health benefits attributed to turmeric/curcumin spice has resulted in its wide utilization as a dietary supplement for companion pets and other animals including horses. While the quantification of free curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and their phase-2 metabolites (curcumin- O -sulfate, curcumin- O -glucuronide) have been extensively investigated in human and rodent biological samples (primarily plasma and serum), there is lack of similar data for horses. Herein, we report a validated LC-ESI–MS/MS method for the simultaneous quantification of the aforementioned free curcuminoids and their metabolites in equine plasma. The linearity of the aforementioned curcuminoids and curcumin- O -sulfate was in the range of 0.5–1000 ng/mL and 1–1000 ng/mL for curcumin- O -glucuronide with 85–115% accuracy and <15% precision in equine plasma. The method was validated based on US FDA criteria and applied to characterize the pharmacokinetics of curcumin- O -sulfate in equine plasma. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07317085
- Volume :
- 154
- Database :
- Academic Search Index
- Journal :
- Journal of Pharmaceutical & Biomedical Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 129095246
- Full Text :
- https://doi.org/10.1016/j.jpba.2018.03.014