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96-Well liquid–liquid extraction liquid chromatography-tandem mass spectrometry method for the quantitative determination of ABT-578 in human blood samples
- Source :
-
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences . Jun2004, Vol. 805 Issue 1, p67. 9p. - Publication Year :
- 2004
-
Abstract
- We report here a quantitative method for the analysis of ABT-578 in human whole blood samples. Sample preparation was achieved by a semi-automated 96-well format liquid–liquid extraction (LLE) method. Aluminum/polypropylene heat seal foil was used to enclose each well of the 96-well plate for the liquid–liquid extraction. A liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) method with pre-column regeneration was developed for the analysis of sample extracts. Selective reaction monitoring (SRM) of the mass transitions m/z 983–935 and m/z 931–883 was employed for the detection of ABT-578 and internal standard, respectively. The ammonium adduct ions [M + NH4]+ generated from electrospray ionization were monitored as the precursor ions. The assay was validated for a linear dynamic range of 0.20–200.75 ng/ml. The correlation coefficient (r) was between 0.9959 and 0.9971. The intra-assay CV (%) was between 1.9 and 13.5% and the inter-assay CV (%) was between 4.7 and 11.3%. The inter-assay mean accuracy was between 86.4 and 102.5% of the theoretical concentrations. [Copyright &y& Elsevier]
- Subjects :
- *BLOOD
*LIQUID chromatography
*MASS spectrometry
*IONS
*AMMONIUM
Subjects
Details
- Language :
- English
- ISSN :
- 15700232
- Volume :
- 805
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 12895911
- Full Text :
- https://doi.org/10.1016/j.jchromb.2004.02.014