沉默ADAM17基因对人甲状腺乳头状癌 细胞增殖、迁移及侵袭的影响.

Objective To study the effects of silencing ADAM 17 on the proliferation, migration, and invasion of human thyroid papillary carcinoma K1 cells. Methods K1 cells were divided into the control group, nonsense shRNA group, and ADAM17-shRNA group. Nonsense-shRNA and ADAM17-shRNA were transfected into K1 cells of the nonsense shRNA group and ADAM17-shRNA group by lentiviral transfection technology. The same amount of PBS was added into the control group. The expression levels of ADAM 17 were detected by RT-PCR and Western blotting. MTT assay and flow cytometry were used to assess the cell proliferation ability and the cell cycle. The cell migration and invasion abilities were detected by Transwell migration and invasion assay, respectively. Results Compared with the control group and nonsense-shRNA group, the mRNA and protein expression levels of ADAM 17 significantly decreased in the ADAM17-shRNA group(all P < 0. 05) ; the optical density(OD value) of the ADAM17-shRNA group at 24 , 48 , and 72 h was significantly lower that that of the control group and nonsense-shRNA group (all P < 0. 05) ; in the ADAM17-shRNA group, the cells arrested in G0/G, phase, and significant difference was found between the ADAM 17-shRNA group and the control group and nonsense-shRNA group (all P <0.05) ; compared with the control group and nonsense-shRNA group, the transmembrane cells decreased sharply in the ADAM 17-shRNA group (all P <0.05). Conclusion Silencing ADAM 17 can inhibit the proliferation, migration, and invasion of K1 cells. [ABSTRACT FROM AUTHOR]