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Sensitive paper-based analytical device for fast colorimetric detection of nitrite with smartphone.
- Source :
-
Analytical & Bioanalytical Chemistry . Apr2018, Vol. 410 Issue 11, p2665-2669. 5p. - Publication Year :
- 2018
-
Abstract
- On-site rapid monitoring of nitrite as an assessment indicator of the environment, food, and physiological systems has drawn extensive attention. Here, electrokinetic stacking (ES) was combined with colorimetric reaction on a paper-based device (PAD) to achieve colorless nitrite detection with smartphone. In this paper, nitrite was stacked on the paper fluidic channel as a narrow band by electrokinetic stacking. Then, Griess reagent was introduced to visualize the stacking band. Under optimal conditions, the sensitivity of nitrite was 160-fold increased within 5 min. A linear response in the range of 0.075 to 1.0 μg mL−1 (<italic>R</italic>2 = 0.99) and a limit of detection (LOD) of 73 ng mL−1 (0.86 μM) were obtained. The LOD was 10 times lower than the reported PAD, and close to that achieved by a desktop spectrophotometer. The applicability was demonstrated by nitrite detection from saliva and water with good selectivity, adding 100 times more concentrated co-ions. High recovery (91.0~108.7%) and reasonable intra-day and inter-day reproducibility (RSD < 9%) were obtained. This work shows that the sensitivity of colorless analyte detection-based colorimetric reaction can be effectively enhanced by integration of ES on a PAD.Schematic of the experimental setups (left) and the corresponding images (right) of the actual portable device.<graphic></graphic> [ABSTRACT FROM AUTHOR]
- Subjects :
- *NITRITES
*ELECTROKINETICS
*SMARTPHONES
*COLORIMETRIC analysis
*ANALYTICAL chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 410
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 128908384
- Full Text :
- https://doi.org/10.1007/s00216-018-0965-2