Back to Search Start Over

Characterization of a fatty acid binding protein from the swimming crab <italic>Portunus trituberculatus</italic> and its effects on the composition of fatty acids in different tissues.

Authors :
Tang, Yujie
Li, Ye
Zhang, Dijun
Su, Xiurong
Zhang, Diya
Source :
Invertebrate Reproduction & Development. Mar2018, Vol. 62 Issue 1, p19-26. 8p.
Publication Year :
2018

Abstract

Fatty acid binding proteins (FABPs) belonging to the lipocalin protein family, have an affinity for fatty acids (FAs) and participate in both the transport of FAs and lipid metabolism. In this study, a 909-bp full-length pt-FABP cDNA containing a 411-bp open reading frame (ORF) was obtained from the swimming crab &lt;italic&gt;Portunus trituberculatus&lt;/italic&gt;. Quantitative real-time PCR revealed that during larval development, pt-FABP expression increased gradually from the zoea to the megalopa, and decreased in the juvenile crab. pt-FABP mRNA was detected at the highest level in the testis, followed by the muscles, with the lowest level in the hepatopancreas. The composition of fatty acids in three different tissues from crabs of different sex was detected by gas chromatography-mass spectrometry (GC-MS). The results indicated that the FA profile was in accordance with the expression of pt-FABP, which suggested that pt-FABP participates in the unsaturated fatty acid transportation and storage system of &lt;italic&gt;P. trituberculatus&lt;/italic&gt;. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
07924259
Volume :
62
Issue :
1
Database :
Academic Search Index
Journal :
Invertebrate Reproduction & Development
Publication Type :
Academic Journal
Accession number :
128907415
Full Text :
https://doi.org/10.1080/07924259.2017.1364674