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Upregulation of α-enolase protects cardiomyocytes from phenylephrine-induced hypertrophy.
- Source :
-
Canadian Journal of Physiology & Pharmacology . 2018, Vol. 96 Issue 4, p352-358. 7p. 2 Charts, 5 Graphs. - Publication Year :
- 2018
-
Abstract
- Cardiac hypertrophy often refers to the abnormal growth of heart muscle through a variety of factors. The mechanisms of cardiomyocyte hypertrophy have been extensively investigated using neonatal rat cardiomyocytes treated with phenylephrine. α-Enolase is a glycolytic enzyme with 'multifunctional jobs' beyond its catalytic activity. Its possible contribution to cardiac dysfunction remains to be determined. The present study aimed to investigate the change of α-enolase during cardiac hypertrophy and explore its role in this pathological process. We revealed that mRNA and protein levels of α-enolase were significantly upregulated in hypertrophic rat heart induced by abdominal aortic constriction and in phenylephrine-treated neonatal rat cardiomyocytes. Furthermore, knockdown of α-enolase by RNA interference in cardiomyocytes mimicked the hypertrophic responses and aggravated phenylephrine-induced hypertrophy without reducing the total glycolytic activity of enolase. In addition, knockdown of α-enolase led to an increase of GATA4 expression in the normal and phenylephrine-treated cardiomyocytes. Our results suggest that the elevation of α-enolase during cardiac hypertrophy is compensatory. It exerts a catalytic independent role in protecting cardiomyocytes against pathological hypertrophy. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00084212
- Volume :
- 96
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Canadian Journal of Physiology & Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 128721814
- Full Text :
- https://doi.org/10.1139/cjpp-2017-0282