An Inexpensive and Convenient Method to Culture Facultative Anaerobic Microorganisms from Yogurt.

It is often difficult for students grasp the concept that some organisms can grow in the absence of oxygen and that oxygen can be toxic to organisms. However, as educators usually describe in the classroom, the importance of the anaerobic growth of microorganisms can be seen in many areas of industry, health, and the creation of several categories of food ranging from fermented alcohols to breads and cheeses. To demonstrate that microorganisms can grow and thrive in the absence of oxygen, we have developed an inexpensive and easy technique to culture facultative anaerobic bacteria from yogurt in high school or undergraduate biology laboratories. Using the method discussed, which uses readily available and low-cost equipment, students can culture and visually identify facultative anaerobic bacteria that they may consume on a regular basis, demonstrating the utility of organisms that thrive in an anaerobic environment. [ABSTRACT FROM AUTHOR]