The first crystal structure of manganese superoxide dismutase from the genus <italic>Staphylococcus</italic>.

A recombinant <italic>Staphylococcus equorum</italic> manganese superoxide dismutase (MnSOD) with an Asp13Arg substitution displays activity over a wide range of pH, at high temperature and in the presence of chaotropic agents, and retains 50% of its activity after irradiation with UVC for up to 45 min. Interestingly, <italic>Bacillus subtilis</italic> MnSOD does not have the same stability, despite having a closely similar primary structure and thus presumably also tertiary structure. Here, the crystal structure of <italic>S. equorum</italic> MnSOD at 1.4 Å resolution is reported that may explain these differences. The crystal belonged to space group <italic>P</italic>3221, with unit‐cell parameters <italic>a</italic> = 57.36, <italic>b</italic> = 57.36, <italic>c</italic> = 105.76 Å, and contained one molecule in the asymmetric unit. The symmetry operation indicates that the enzyme has a dimeric structure, as found in nature and in <italic>B. subtilis</italic> MnSOD. As expected, their overall structures are nearly identical. However, the loop connecting the helical and α/β domains of <italic>S. equorum</italic> MnSOD is shorter than that in <italic>B. subtilis</italic> MnSOD, and adopts a conformation that allows more direct water‐mediated hydrogen‐bond interactions between the amino‐acid side chains of the first and last α‐helices in the latter domain. Furthermore, <italic>S. equorum</italic> MnSOD has a slightly larger buried area compared with the dimer surface area than that in <italic>B. subtilis</italic> MnSOD, while the residues that form the interaction in the dimer‐interface region are highly conserved. Thus, the stability of <italic>S. equorum</italic> MnSOD may not originate from the dimeric form alone. Furthermore, an additional water molecule was found in the active site. This allows an alternative geometry for the coordination of the Mn atom in the active site of the apo form. This is the first structure of MnSOD from the genus <italic>Staphylococcus</italic> and may provide a template for the structural study of other MnSODs from this genus. [ABSTRACT FROM AUTHOR]