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Identification of Arcanobacterium phocae isolated from fur animals by phenotypic properties, by MALDI-TOF MS analysis and by detection of phocaelysin encoding gene phl as probable novel target.

Identification of Arcanobacterium phocae isolated from fur animals by phenotypic properties, by MALDI-TOF MS analysis and by detection of phocaelysin encoding gene phl as probable novel target.

Authors :
Alssahen, Mazen
Sammra, Osama
Wickhorst, Jörn-Peter
Hassan, Abdulwahed Ahmed
Lämmler, Christoph
Saarnisto, Mirja Raunio
Prenger-Berninghoff, Ellen
Timke, Markus
Becker, Andrè
Abdulmawjood, Amir
Source :
Veterinary Microbiology. Mar2018, Vol. 216, p45-51. 7p.
Publication Year :
2018

Abstract

In the present study 12 Arcanobacterium phocae strains isolated from fur animals in Finland, including foxes, minks and Finnraccoons, could successfully be identified phenotypically, by matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and phocaelysin (PHL) encoding gene phl . The PHL of all 12 A. phocae strains in the present study and reference strains A. phocae DSM 10002 T and A. phocae DSM 10003 displayed, as typical members of the cholesterol dependent cytolysin-group of toxins, the variant undecapeptide sequence EATGLAWDPWW which appeared to be most closely related to arcanolysin of Arcanobacterium haemolyticum and pyolysin of Trueperella pyogenes . In addition, gene phl could be determined with a newly designed loop-mediated isothermal amplification (LAMP) assay. The detection of mass spectra by MALDI-TOF MS and the LAMP assay based on gene phl might help to reliably identify A. phocae in future and also elucidate the role this species plays in infections of fur animals. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03781135
Volume :
216
Database :
Academic Search Index
Journal :
Veterinary Microbiology
Publication Type :
Academic Journal
Accession number :
128303147
Full Text :
https://doi.org/10.1016/j.vetmic.2018.01.017