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MiR-29 family members interact with SPARC to regulate glucose metabolism.
- Source :
-
Biochemical & Biophysical Research Communications . Mar2018, Vol. 497 Issue 2, p667-674. 8p. - Publication Year :
- 2018
-
Abstract
- MicroRNA (miR)-29 family members have been reported to play important regulatory roles in metabolic disease. We used TargetScan to show that “secreted protein acidic rich in cysteine” (SPARC) is a target of the miR-29s. SPARC is a multifunctional secretory protein involved in a variety of biological activities, and SPARC dysregulation is associated with a wide range of obesity-related disorders, including type 2 diabetes mellitus (T2DM). We explored whether miR-29s played roles in glucose metabolism and whether miR-29s directly targeted SPARC. We also examined the effect of SPARC on glucose metabolism and how the association of miR-29s with SPARC affected glucose metabolism. We found that overexpression of miR-29s reduced glucose uptake and GLUT4 levels; that miR-29 directly targeted SPARC, resulting in degradation of SPARC-encoding mRNA and reduction in the SPARC protein level; that SPARC increased glucose uptake and GLUT4 levels; that shRNA-mediated knockdown of SPARC reduced GLUT4 protein levels in 3T3-L1 adipocytes; that miR-29s reduced glucose uptake and GLUT4 levels; and that miR-29s inhibited glucose uptake by suppressing SPARC synthesis. Thus, the miR-29 family negatively regulates glucose metabolism by inhibiting SPARC expression. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICRORNA
*GLUCOSE metabolism
*METABOLIC disorders
*CYSTEINE
*TYPE 2 diabetes
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 497
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 128275026
- Full Text :
- https://doi.org/10.1016/j.bbrc.2018.02.129