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Pancreatic-like enzymes of microbial origin restore growth and normalize lipid absorption in a pig model with exocrine pancreatic insufficiency.

Authors :
Pierzynowska, Kateryna
Valverde-Piedra, Jose
Szymanczyk, Sylwia
Prykhod'ko, Olena
Pieszka, Marek
Kardas, Marek
Grochowska-Niedworok, Elżbieta
Grabowski, Tomasz
Winiarczyk, Mateusz
Pierzynowski, Stefan
Source :
Archives of Medical Science. Mar2018, Vol. 14 Issue 2, p407-414. 8p.
Publication Year :
2018

Abstract

<bold>Introduction: </bold>The standard therapy for exocrine pancreatic insufficiency (EPI) is porcine-derived pancreatic enzyme replacement therapy (PERT). In the present study we tested a new approach with a mixture of pancreatic-like enzymes of microbial origin (PLEM) in a 1-week efficacy study in EPI pigs. In addition to the conventionally used coefficient of fat and nitrogen absorption (CFA and CNA), parameters that more accurately reflect the nutritional and health status, such as changes in the lipemic index (LI), plasma triglyceride (TG) and non-esterified fatty acid (NEFA) levels, and somatic growth, were determined.<bold>Material and Methods: </bold>A PLEM dose containing 120 000 active lipase units, 80 000 active protease units and 12 000 active amylase units (all from Sigma, St. Louis, MO) was given as a powder, twice daily with a meal (40 g fat/meal) to 8 EPI pigs for 7 days. Ten healthy pigs were used as a comparator.<bold>Results: </bold>The PLEM enhanced fat and protein digestion, and reversed growth impairment in EPI pigs. With treatment, CFA and CNA increased by 59% and 43% (p < 0.05), respectively. Although fat and protein absorption were lower than in the comparator, the postprandial blood lipid profile was normal as in healthy pigs. The mucosal thickness significantly increased by 27%, 50% and 26%, in the proximal, middle, and distal jejunum (p < 0.05) with treatment and resembled that of healthy animals.<bold>Conclusions: </bold>Pancreatic-like enzymes of microbial origin supported somatic growth and normalized the postprandial lipid profile. As a measure of efficacy, postprandial LI, TG and NEFA are viable endpoints to be explored in human trials. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17341922
Volume :
14
Issue :
2
Database :
Academic Search Index
Journal :
Archives of Medical Science
Publication Type :
Academic Journal
Accession number :
128142840
Full Text :
https://doi.org/10.5114/aoms.2018.73471