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Dendritic core-shell silica spheres with large pore size for separation of biomolecules.
- Source :
-
Journal of Chromatography A . Mar2018, Vol. 1540, p31-37. 7p. - Publication Year :
- 2018
-
Abstract
- Monodispersed core-shell silica spheres with fibrous shell structure and tunable pore size were prepared by using a one-pot oil-water biphase method. The pore size could be tuned from 7 nm to 37 nm by using organic solvents with different polarities as oil phase. The spheres synthesized by using benzene as organic solvent had the maximum pore size of 37 nm and possessed a surface area of 61 m 2 g −1 . The obtained wide pore core-shell silica spheres were applied for rapidly separating small molecules, peptides, small proteins, and large proteins with molecular weight up to 200 kDa. Since the pore size of the core-shell silica spheres was sufficiently large for the free access of all the solutes, sharp and symmetric peaks were obtained. The separation performance was as high as 264,531 plates m −1 for fluorene. The great efficient separation demonstrates that the wide pore core-shell silica spheres have a great potential for rapid analysis of both small and large solutes with high performance liquid chromatography. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219673
- Volume :
- 1540
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography A
- Publication Type :
- Academic Journal
- Accession number :
- 128092501
- Full Text :
- https://doi.org/10.1016/j.chroma.2018.02.002