Back to Search
Start Over
Methods for lineage tracing on the organism-wide level.
- Source :
-
Current Opinion in Cell Biology . Dec2017, Vol. 49, p16-21. 6p. - Publication Year :
- 2017
-
Abstract
- Determining the lineage origin of cell types is a major goal in developmental biology. Furthermore, lineage tracing is a powerful approach for understanding the origin of developmental defects as well as the origin of diseases such as cancer. There is now a variety of complementary approaches for identifying lineage relationships, ranging from direct observation of cell divisions by light microscopy to genetic labeling of cells using inducible recombinases and fluorescent reporters. A recent development, and the main topic of this review article, is the use of high-throughput sequencing data for lineage analysis. This emerging approach holds the promise of increased multiplexing capacity, allowing lineage analysis of large cell numbers up to the organism-wide level combined with simultaneous transcription profiling by single cell RNA sequencing. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09550674
- Volume :
- 49
- Database :
- Academic Search Index
- Journal :
- Current Opinion in Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- 128073737
- Full Text :
- https://doi.org/10.1016/j.ceb.2017.11.004