Green synthesized PLA/silver nanoparticle probe for sensing of hydrogen peroxide in biological samples.

In this paper, the physio-chemical characteristics of green synthesised PLA/silver nanoparticles (PLA-AgNPs) system was investigated for H 2 O 2 sensing. The precursor, silver nitrate (AgNO 3 ) was reduced to nanosilver (n-Ag) by Piper nigrum leaves extract in dual phase medium containing PLA (Capping agent). X-ray photo electron spectroscopy (XPS) analysis was carried out to evaluate the interaction of PLA with silver nanoparticles. The morphological analysis by Transmission electron microscopy (TEM) obtained images of spherical silver particles with average size of about 10–20 nm. The H 2 O 2 sensing was confirmed from the colour fading of the solution and vanishing of the characteristic surface plasmon resonance (SPR) absorbance peak of PLA-AgNPs on adding H 2 O 2 . The proposed sensor can detect even very low concentrations (5 μM) of hydrogen peroxide and finds applicability in biological and environmental analysis. [ABSTRACT FROM AUTHOR]