Proteine aus <em>neurospora crassa</em> 1. Immunchemische Analyse cytoplasmatischer Proteine aus <em>Neurospora crassa</em> 3a6A und verschiedenen Mutanten.

The mycelium of Neurospora crassa was cultivated in a minimal liquid medium in surface culture. The moist mycelium was lyophilized, powdered, and eluted using 0,14 M sodium chloride solution for extracting the proteins. The enrichment of these proteins was carried out by means of ultrafiltration (vacuum and pressure filtration). The extracted proteins of N. crassa were characterized by paper-, agar gel- and immuno&#173;electrophoresis. The antisera were obtained by immunising rabbits for 21 weeks. By means of agar gel electrophoresis 5 zones of proteins were separated in N. crassa 3a6A; by means of immunoelectrophoresis 17 bands resulted from the same protein mixture. The electrophoretic mobilities of the precipitation arcs were calculated. The immunoelectrophoretic patterns of N. crassa E 5256 obtained by N. crassa 3a6A antiserum nearly agreed with the bands of N. crassa 3a6A antigen. However there were no corresponding results in the patterns obtained from N. crassa 46001-1-1a and N. crassa 6994a with the same antiserum. It may be assumed that these differences depend on the different genetic enzyme patterns of the wild and mutant strains; they are also responsible for the differences in the rates of synthesis depending on the duration of cultivation. [ABSTRACT FROM AUTHOR]