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Host-based lipid inflammation drives pathogenesis in Francisella infection.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America . 11/21/2017, Vol. 114 Issue 47, p12596-12601. 6p. - Publication Year :
- 2017
-
Abstract
- Mass spectrometry imaging (MSI) was used to elucidate host lipids involved in the inflammatory signaling pathway generated at the host-pathogen interface during a septic bacterial infection. Using Francisella novicida as a model organism, a bacterial lipid virulence factor (endotoxin) was imaged and identified along with host phospholipids involved in the splenic response in murine tissues. Here, we demonstrate detection and distribution of endotoxin in a lethal murine F. novicida infection model, in addition to determining the temporally and spatially resolved innate lipid inflammatory response in both 2D and 3D renderings using MSI. Further, we show that the cyclooxygenase-2-dependent lipid inflammatory pathway is responsible for lethality in F. novicida infection due to overproduction of proinflammatory effectors including prostaglandin E2. The results of this study emphasize that spatial determination of the host lipid components of the immune response is crucial to identifying novel strategies to effectively address highly pathogenic and lethal infections stemming from bacterial, fungal, and viral origins. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FRANCISELLA
*INFLAMMATION
*GRAM-negative bacteria
*PATHOLOGY
*LIPIDS
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 114
- Issue :
- 47
- Database :
- Academic Search Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 127823129
- Full Text :
- https://doi.org/10.1073/pnas.1712887114