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The role of intermediate filaments in maintaining integrity and function of intestinal epithelial cells after massive bowel resection in a rat.
- Source :
-
Pediatric Surgery International . Feb2018, Vol. 34 Issue 2, p217-225. 9p. 1 Diagram, 2 Charts, 2 Graphs. - Publication Year :
- 2018
-
Abstract
- <bold>Purpose: </bold>Intermediate filaments (IFs) are a part of the cytoskeleton that extend throughout the cytoplasm of all cells and function in the maintenance of cell-shape by bearing tension and serving as structural components of the nuclear lamina. In normal intestine, IFs provide a tissue-specific three-dimensional scaffolding with unique context-dependent organizational features. The purpose of this study was to evaluate the role of IFs during intestinal adaptation in a rat model of short bowel syndrome (SBS).<bold>Materials and Methods: </bold>Male rats were divided into two groups: Sham rats underwent bowel transection and SBS rats underwent a 75% bowel resection. Parameters of intestinal adaptation, enterocyte proliferation and apoptosis were determined 2 weeks after operation. Illumina's Digital Gene Expression (DGE) analysis was used to determine the cytoskeleton-related gene expression profiling. IF-related genes and protein expression were determined using real-time PCR, Western blotting and immunohistochemistry.<bold>Results: </bold>Massive small bowel resection resulted in a significant increase in enterocyte proliferation and concomitant increase in cell apoptosis. From the total number of 20,000 probes, 16 cytoskeleton-related genes were investigated. Between these genes, only myosin and tubulin levels were upregulated in SBS compared to sham animals. Between IF-related genes, desmin, vimentin and lamin levels were down-regulated and keratin and neurofilament remain unchanged. The levels of TGF-β, vimentin and desmin gene and protein were down-regulated in resected rats (vs sham animals).<bold>Conclusions: </bold>Two weeks following massive bowel resection in rats, the accelerated cell turnover was accompanied by a stimulated microfilaments and microtubules, and by inhibited intermediate filaments. Resistance to cell compression rather that maintenance of cell-shape by bearing tension are responsible for contraction, motility and postmitotic cell separation in a late stage of intestinal adaptation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *INTESTINAL disease treatment
*EPITHELIAL cells
*SURGICAL excision
*INTESTINAL surgery
*CYTOPLASMIC filaments
*LABORATORY rats
*SMALL intestine surgery
*ANIMAL experimentation
*APOPTOSIS
*BIOLOGICAL models
*CELL physiology
*CYTOPLASM
*CYTOSKELETAL proteins
*GENES
*IMMUNOHISTOCHEMISTRY
*SMALL intestine
*POLYMERASE chain reaction
*PROTEINS
*RATS
*RNA
*WESTERN immunoblotting
*REVERSE transcriptase polymerase chain reaction
*SHORT bowel syndrome
DIGESTIVE organ surgery
Subjects
Details
- Language :
- English
- ISSN :
- 01790358
- Volume :
- 34
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Pediatric Surgery International
- Publication Type :
- Academic Journal
- Accession number :
- 127735475
- Full Text :
- https://doi.org/10.1007/s00383-017-4192-2