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Picroside I and Picroside II from Tissue Cultures of Picrorhiza kurroa.
- Source :
-
Pharmacognosy Research . 2017 Supplement 1, Vol. 9, pS53-S56. 4p. - Publication Year :
- 2017
-
Abstract
- Background: Picrorhiza kurroa (PK) belongs to Scrophulariaceae family and is a representative endemic, medicinal herb, widely distributed throughout the higher altitudes of alpine Himalayas from west to east, between 3000 and 4500 m above mean sea level. Objective: The objective of the present study is to assess the production of picroside I and picroside II from tissue cultures of PK. Materials and Methods: Auxiliary shoot tips of PK were incubated in Murashige and Skoog medium supplemented with indole-3-butyric acid and kinetin phytohormones. The callus produced was collected at different time intervals and was processed for extraction of picroside I and picroside II followed by thin layer chromatography and high-performance liquid chromatography HPLC analysis. Results: The maximum growth index was found to be 5.109 ± 0.159 at 16-week-old callus culture. The estimation of picroside-I and picroside-II was carried out by (HPLC) analysis; quantity of secondary metabolite found to be 16.37 ± 0.0007 mg/g for PK-I and 6.34 ± 0.0012 mg/g for PK-II. Conclusion: This is the first attempt to produce the Picroside-I and II in large amount by the tissue culture technique. It can be observed that the method of callus culture can be used in production of secondary metabolites Picroside-I and II from PK [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09764836
- Volume :
- 9
- Database :
- Academic Search Index
- Journal :
- Pharmacognosy Research
- Publication Type :
- Academic Journal
- Accession number :
- 127711018
- Full Text :
- https://doi.org/10.4103/pr.pr_89_17