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Three-dimensional reconstruction with serial whole-mount sections of oral tongue squamous cell carcinoma: A preliminary study.
- Source :
-
Journal of Oral Pathology & Medicine . Jan2018, Vol. 47 Issue 1, p53-59. 7p. 4 Color Photographs, 1 Chart. - Publication Year :
- 2018
-
Abstract
- <bold>Objectives: </bold>Margin status and invasion pattern are prognostic factors for oral tongue squamous cell carcinoma (OTSCC). Current methods to identify these factors are limited to 2D observation; it is necessary to explore 3D reconstruction with whole-mount sample to improve the accuracy of analysis. This study aimed to study the tissue preparation, section generation, and 3D reconstruction with whole-mount OTSCC specimen.<bold>Study Design: </bold>Two OTSCC samples were retrieved from Nanjing Stomatological Hospital, Medical School of Nanjing University. One sample was sliced into 3 equal-sized pieces and subjected to different processing schedules to determine the best method. The second sample was processed accordingly. Serial whole-mount sections of the second sample were generated, stained with HE/anticytokine antibody in intersection manner, and scanned into digital images. Digital images were aligned and reconstructed into 3D images with Hetero Genius Medical Image Manager 3D Pathology Add-On [HGMIM3D].<bold>Results: </bold>Successful serial whole-mount sections of comparable quality to traditional sections were generated. Three-dimensional images with serial whole-mount sections were successfully generated.<bold>Conclusions: </bold>Whole-mount histopathological 3D reconstruction of OTSCC was successfully generated, providing a solid foundation for comprehensive margin and invasion analysis. Although future study and improvement were needed, whole-mount histopathological 3D reconstruction proved to be a promising method in OTSCC study. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09042512
- Volume :
- 47
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Oral Pathology & Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 127566573
- Full Text :
- https://doi.org/10.1111/jop.12644