VEGF-C在口腔颌面部淋巴管瘤组织中表达变化 及其对人脐静脉内皮细胞增殖的影响

Objective To explore the expression of vascular endothelial growth factor-C ( VEGF-C) in the occurrence and development of oral and maxillofacial lymphangioma (OML). Methods The tumor tissues (OML group) and the adjacent normal tissue (control group) from OML patients were collected. The real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of VEGF-C mRNA in the tissues. Immunohistochemistry (IHC) was used to detect the positive rate of VEGF-C protein expression in the tissues. ECV304 cells cultured in vitro were randomly divided into two groups: the observation group which was transfected with sh-VEGF-C plasmid and the control group transfected with control plasmid sh-control. After 48-hour transfection, qRT-PCR and Western blotting were used to detect the relative expression of VEGF-C protein, and MTT assay was used to detect the proliferation of the cells (absorbance value). Results In the OML group and control group, the expression of VEGF-C mRNA was 1.061 ±0.284 and 0.801 ±0.098, respectively; the positive expression rates of VEGF-C protein was 84. 10% (42/54) and 10. 13% (5/54) , respectively ( P <0. 05 or P < 0. 01 ). In the observation group and control group, the relative expression levels of VEGF-C mRNA were 0. 200 ±0. 058 and 0. 967 ±0. 017, respectively; the relative expression levels of VEGF-C protein were 0. 319 ±0. 021 and 0.965 ±0.039, respectively; the A values were 0. 393 ±0.015 and 0. 97 ±0. 004, respectively; significant differences were found between these two groups (all P <0.01 ). Conclusions VEGF-C is highly expressed in the OML tissues, and decreasing VEGF-C can inhibit the proliferation of human umbilical vein endothelial cells. [ABSTRACT FROM AUTHOR]