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MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model.

Authors :
YULEI DONG
TAO LI
YULONG LI
SHAODA REN
JUNFEN FAN
XISHENG WENG
Source :
Molecular Medicine Reports. Dec2017, Vol. 16 Issue 6, p9331-9336. 6p.
Publication Year :
2017

Abstract

The mechanism of steroid-associated femoral head necrosis remains unclear. The present study investigated the role of microRNA-23a-3p (miR-23a-3p) in the incidence of osteonecrosis in a rat model. An miR-23a-3p mimic, an inhibitor and a negative control were transfected into bone mesenchymal stem cells using a lentiviral vector, and then injected into the steroid-induced femoral head necrosis model. Osteonecrosis incidence was assessed by micro computed tomography and histopathology. Low-density lipoprotein receptor-related protein 5 (LRP-5) expression was assessed by immunohistochemistry. The results demonstrated the incidence of osteonecrosis decreased in the miR-23a-3p inhibitor group compared with the miR-23a-3p mimic group (18.2% vs. 75%; P<0.05). The ratio of bone volume/total volume and trabecular thickness were significantly increased in the miR-23a-3p inhibitor group compared with the miR-23a mimic group. The expression level of LRP-5 was higher in the miR-23a-3p inhibitor group. The present study indicated that miR may provide a novel and alternative approach for understanding the mechanism underlying steroid-associated necrosis of the femoral head. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17912997
Volume :
16
Issue :
6
Database :
Academic Search Index
Journal :
Molecular Medicine Reports
Publication Type :
Academic Journal
Accession number :
127333923
Full Text :
https://doi.org/10.3892/mmr.2017.7808