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MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model.
- Source :
-
Molecular Medicine Reports . Dec2017, Vol. 16 Issue 6, p9331-9336. 6p. - Publication Year :
- 2017
-
Abstract
- The mechanism of steroid-associated femoral head necrosis remains unclear. The present study investigated the role of microRNA-23a-3p (miR-23a-3p) in the incidence of osteonecrosis in a rat model. An miR-23a-3p mimic, an inhibitor and a negative control were transfected into bone mesenchymal stem cells using a lentiviral vector, and then injected into the steroid-induced femoral head necrosis model. Osteonecrosis incidence was assessed by micro computed tomography and histopathology. Low-density lipoprotein receptor-related protein 5 (LRP-5) expression was assessed by immunohistochemistry. The results demonstrated the incidence of osteonecrosis decreased in the miR-23a-3p inhibitor group compared with the miR-23a-3p mimic group (18.2% vs. 75%; P<0.05). The ratio of bone volume/total volume and trabecular thickness were significantly increased in the miR-23a-3p inhibitor group compared with the miR-23a mimic group. The expression level of LRP-5 was higher in the miR-23a-3p inhibitor group. The present study indicated that miR may provide a novel and alternative approach for understanding the mechanism underlying steroid-associated necrosis of the femoral head. [ABSTRACT FROM AUTHOR]
- Subjects :
- *STEROIDS
*HEAD & neck cancer
*MICRORNA
*OSTEONECROSIS
*ANIMAL models in research
Subjects
Details
- Language :
- English
- ISSN :
- 17912997
- Volume :
- 16
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Molecular Medicine Reports
- Publication Type :
- Academic Journal
- Accession number :
- 127333923
- Full Text :
- https://doi.org/10.3892/mmr.2017.7808