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Moloney leukemia virus 10 (MOV10) inhibits the degradation of APOBEC3G through interference with the Vif-mediated ubiquitin-proteasome pathway.
- Source :
-
Retrovirology . 12/19/2017, Vol. 14, p1-19. 19p. - Publication Year :
- 2017
-
Abstract
- Background: MOV10 protein has ATP-dependent 5'-3' RNA helicase activity and belongs to the UPF1p superfamily. It can inhibit human immunodeficiency virus type 1 (HIV-1) replication at multiple stages and interact with apolipoprotein-B-mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G or A3G), a member of the cytidine deaminase family that exerts potent inhibitory effects against HIV-1 infection. However, HIV-1-encoded virion infectivity factor (Vif ) protein specifically mediates the degradation of A3G via the ubiquitin-proteasome system (UPS). Results: We demonstrate that MOV10 counteracts Vif-mediated degradation of A3G by inhibiting the assembly of the Vif-CBF-β-Cullin 5-ElonginB-ElonginC complex. Through interference with UPS, MOV10 enhances the level of A3G in HIV-1-infected cells and virions, and synergistically inhibits the replication and infectivity of HIV-1. In addition, the DEAG-box of MOV10 is required for inhibition of Vif-mediated A3G degradation as the DEAG-box mutant significantly loses this ability. Conclusions: Our results demonstrate a novel mechanism involved in the anti-HIV-1 function of MOV10. Given that both MOV10 and A3G belong to the interferon antiviral system, their synergistic inhibition of HIV-1 suggests that these proteins may play complicated roles in antiviral functions. [ABSTRACT FROM AUTHOR]
- Subjects :
- *UBIQUITIN
*PROTEASOMES
*POLYPEPTIDES
*HIV
*PROTEINS
Subjects
Details
- Language :
- English
- ISSN :
- 17424690
- Volume :
- 14
- Database :
- Academic Search Index
- Journal :
- Retrovirology
- Publication Type :
- Academic Journal
- Accession number :
- 126974546
- Full Text :
- https://doi.org/10.1186/s12977-017-0382-1