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Identification of berberrubine metabolites in rats by using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.
- Source :
-
Fitoterapia . Jan2018, Vol. 124, p23-33. 11p. - Publication Year :
- 2018
-
Abstract
- Berberrubine, an isoquinoline alkaloid isolated from many medicinal plants, possesses diverse pharmacological activities, including glucose-lowering, lipid-lowering, anti-inflammatory, and anti-tumor effects. This study aimed to investigate the metabolic profile of berberrubine in vivo . Therefore, a rapid and reliable method using the ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and metabolynxâ„¢ software with mass defect filter (MDF) technique was developed. Plasma, bile, urine and feces samples were collected from rats after oral administration of berberrubine with a dose of 30.0 mg/kg and analyzed to characterize the metabolites of berberrubine in vivo for the first time. A total of 57 metabolites were identified, including 54 metabolites in urine, 39 metabolites in plasma, 28 metabolites in bile and 18 metabolites in feces. The results indicated that demethylenation, reduction, hydroxylation, demethylation, glucuronidation, and sulfation were the major metabolic pathways of berberrubine in vivo . [ABSTRACT FROM AUTHOR]
- Subjects :
- *FECAL analysis
*ALKALOIDS
*ALTERNATIVE medicine
*ANIMAL experimentation
*BILE
*HIGH performance liquid chromatography
*HYDROXYLATION
*MASS spectrometry
*MEDICINAL plants
*METHYLATION
*ORAL drug administration
*OXIDATION-reduction reaction
*RATS
*PLANT extracts
*DATA analysis software
*IN vivo studies
Subjects
Details
- Language :
- English
- ISSN :
- 0367326X
- Volume :
- 124
- Database :
- Academic Search Index
- Journal :
- Fitoterapia
- Publication Type :
- Academic Journal
- Accession number :
- 126946257
- Full Text :
- https://doi.org/10.1016/j.fitote.2017.10.005