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Immobilization of Saccharomyces cerevisiae using polyethyleneimine grafted collagen fibre as support and investigations of its fermentation performance.
- Source :
-
Biotechnology & Biotechnological Equipment . Feb2018, Vol. 32 Issue 1, p109-115. 7p. - Publication Year :
- 2018
-
Abstract
- In the present investigation, an collagen fibre (CF), abundant natural biomass, was successfully grafted by polyethyleneimine (PEI). The resultant PEI grafted collagen fibre (CF-PEI) was employed as biocompatible and high cell loading support matrix for the immobilization of Saccharomyces cerevisiae cells. The as-prepared CF-PEI immobilized cells (CF-PEI-cell) exhibited high activity and stability for both batch and continuous fermentation. In batch fermentation, CF-PEI-cells showed enhanced stability as compared with other matrices supported cells, and produced an average ethanol concentration of 45.04 g/L with ethanol yield (YP/S) of 0.46 g/g and glucose conversion efficiency (η) of 90.4%. Continuous fermentation was operated stably in a down-flow trickling bed reactor charged with CF-PEI-cell for a total of 2 months. When the dilution rate was 0.16 1/h, the average ethanol productivity reached 7.18 g/(L h) with η of 88.94%. Further scanning electron microscopy observations confirmed that yeast cells can proliferate on the surface of CF-PEI during ethanol fermentation, which demonstrates that CF-PEI is indeed an ideal matrix for the immobilization of yeast cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13102818
- Volume :
- 32
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biotechnology & Biotechnological Equipment
- Publication Type :
- Academic Journal
- Accession number :
- 126917842
- Full Text :
- https://doi.org/10.1080/13102818.2017.1389302