Back to Search Start Over

Surface immobilization of gelatin onto TiO2 nanotubes to modulate osteoblast behavior.

Authors :
Lai, Min
Jin, Ziyang
Qiao, Weili
Source :
Colloids & Surfaces B: Biointerfaces. Nov2017, Vol. 159, p743-749. 7p.
Publication Year :
2017

Abstract

To improve the bioactivity of titanium implants, a homogeneous layer of TiO 2 nanotubes with a diameter of approximately 110 nm was prepared by anodization. Gelatin was immobilized onto TiO 2 nanotubes through an intermediate layer of polydopamine. The surface characteristics of different substrates were evaluated using X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and contact angle measurements, respectively. These results demonstrate that gelatin was successfully immobilized onto TiO 2 nanotubes. In vitro cell culture experiments including immunofluorescence staining, cell viability, alkaline phosphatase (ALP), mineralization and the expression of osteogenic genes including runt-related transcription factor 2 (Runx2), ALP, collagen type I (Col I), and osteopontin (OPN) confirm that cell spreading, proliferation and osteoblastic differentiation were improved when cells were seeded onto gelatin-immobilized TiO 2 nanotubes. This resulting material shows great promise as a future material in titanium implant applications. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09277765
Volume :
159
Database :
Academic Search Index
Journal :
Colloids & Surfaces B: Biointerfaces
Publication Type :
Academic Journal
Accession number :
126896615
Full Text :
https://doi.org/10.1016/j.colsurfb.2017.08.043