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Surface immobilization of gelatin onto TiO2 nanotubes to modulate osteoblast behavior.
- Source :
-
Colloids & Surfaces B: Biointerfaces . Nov2017, Vol. 159, p743-749. 7p. - Publication Year :
- 2017
-
Abstract
- To improve the bioactivity of titanium implants, a homogeneous layer of TiO 2 nanotubes with a diameter of approximately 110 nm was prepared by anodization. Gelatin was immobilized onto TiO 2 nanotubes through an intermediate layer of polydopamine. The surface characteristics of different substrates were evaluated using X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and contact angle measurements, respectively. These results demonstrate that gelatin was successfully immobilized onto TiO 2 nanotubes. In vitro cell culture experiments including immunofluorescence staining, cell viability, alkaline phosphatase (ALP), mineralization and the expression of osteogenic genes including runt-related transcription factor 2 (Runx2), ALP, collagen type I (Col I), and osteopontin (OPN) confirm that cell spreading, proliferation and osteoblastic differentiation were improved when cells were seeded onto gelatin-immobilized TiO 2 nanotubes. This resulting material shows great promise as a future material in titanium implant applications. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09277765
- Volume :
- 159
- Database :
- Academic Search Index
- Journal :
- Colloids & Surfaces B: Biointerfaces
- Publication Type :
- Academic Journal
- Accession number :
- 126896615
- Full Text :
- https://doi.org/10.1016/j.colsurfb.2017.08.043