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Site-Specific One-to-One Click Coupling of Single Proteins to Individual Carbon Nanotubes: A Single-Molecule Approach.

Authors :
Freeley, Mark
Worthy, Harley L.
Ahmed, Rochelle
Bowen, Ben
Watkins, Daniel
Macdonald, J. Emyr
Ming Zheng
Jones, D. Dafydd
Palma, Matteo
Source :
Journal of the American Chemical Society. 12/13/2017, Vol. 139 Issue 49, p17834-17840. 7p.
Publication Year :
2017

Abstract

We report the site-specific coupling of single proteins to individual carbon nanotubes (CNTs) in solution and with single-molecule control. Using an orthogonal Click reaction, Green Fluorescent Protein (GFP) was engineered to contain a genetically encoded azide group and then bound to CNT ends in different configurations: in close proximity or at longer distances from the GFP's functional center. Atomic force microscopy and fluorescence analysis in solution and on surfaces at the single-protein level confirmed the importance of bioengineering optimal protein attachment sites to achieve direct protein-nanotube communication and bridging. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00027863
Volume :
139
Issue :
49
Database :
Academic Search Index
Journal :
Journal of the American Chemical Society
Publication Type :
Academic Journal
Accession number :
126761851
Full Text :
https://doi.org/10.1021/jacs.7b07362