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Arabidopsis mTERF6 is required for leaf patterning.
- Source :
-
Plant Science . Jan2018, Vol. 266, p117-129. 13p. - Publication Year :
- 2018
-
Abstract
- To enhance our understanding of the roles of mitochondrial transcription termination factors (mTERFs) in plants, we have taken a reverse genetic approach in Arabidopsis thaliana . One of the mutants isolated carried a novel allele of the mTERF6 gene, which we named mterf6-5 . mTERF6 is a chloroplast and mitochondrial localised protein required for the maturation of chloroplast isoleucine tRNA. The mterf6-5 plants are pale and exhibit markedly reduced growth, and altered leaf and chloroplast development. Our qRT-PCR analyses revealed mis-expression of several plastid, mitochondrial and nuclear genes in mterf6-5 plants. Synergistic phenotypes were observed in double mutant combinations of mterf6-5 with alleles of other mTERF genes as well as with scabra3-2 , affected in the plastid RpoTp RNA polymerase; these observations suggest a functional relationship between mTERF6 , other mTERFs and SCA3 . The mterf6-5 mutation also enhanced the leaf dorsoventral polarity defects of the asymmetric leaves1-1 ( as1-1 ) mutant, which resulted in radial leaves. This interaction seemed specific of the impaired mTERF6 function because mutations in the mTERF genes MDA1 or TWR-1/mTERF9 did not result in radialised leaves. Furthermore, the mterf6-5 mutation dramatically increased the leaf phenotype of as2-1 and caused lethality early in vegetative development. Our results uncover a new role for mTERF6 in leaf patterning and highlight the importance of mTERFs in plant development. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ARABIDOPSIS thaliana
*LEAF anatomy
*PLANT mutation
*PLANT mitochondria
*PLANT genes
Subjects
Details
- Language :
- English
- ISSN :
- 01689452
- Volume :
- 266
- Database :
- Academic Search Index
- Journal :
- Plant Science
- Publication Type :
- Academic Journal
- Accession number :
- 126709232
- Full Text :
- https://doi.org/10.1016/j.plantsci.2017.11.003