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Optimizing Human Induced Pluripotent Stem Cell Expansion in Stirred-Suspension Culture.
- Source :
-
Stem Cells & Development . Dec2017, Vol. 26 Issue 24, p1804-1817. 14p. - Publication Year :
- 2017
-
Abstract
- Human induced pluripotent stem cells (hiPSCs) hold great hopes for application in regenerative medicine due to their inherent capacity to self-renew and differentiate into cells from the three embryonic germ layers. For clinical applications, a large quantity of hiPSCs produced in standardized and scalable culture processes is required. Several groups, including ours, have developed methodologies for scaled-up hiPSC production in stirred bioreactors in chemically defined medium. In this study, we optimized the critical steps and factors that affect hiPSC expansion and yield in stirred-suspension cultures, including inoculation conditions, seeding density, aggregate size, agitation rate, and cell passaging method. After multiple passages in stirred-suspension bioreactors, hiPSCs remained pluripotent, karyotypically normal, and capable of differentiating into all three germ layers. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15473287
- Volume :
- 26
- Issue :
- 24
- Database :
- Academic Search Index
- Journal :
- Stem Cells & Development
- Publication Type :
- Academic Journal
- Accession number :
- 126600896
- Full Text :
- https://doi.org/10.1089/scd.2017.0090