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A validated LC-MS/MS method for the simultaneous determination of 20-( S)-protopanaxatriol and its two active metabolites in rat plasma: Application to a pharmacokinetics study.
- Source :
-
Journal of Separation Science . Dec2017, Vol. 40 Issue 23, p4503-4510. 8p. - Publication Year :
- 2017
-
Abstract
- We present a validated liquid chromatography with tandem mass spectrometry method for simultaneous determination of 20-( S)-protopanaxatriol and its two oxidative stereoisomeric metabolites (20 S,24 S)-epoxy-dammarane-3,6,12,25-tetraol (M1) and (20 S,24 R)-epoxy-dammarane-3,6,12,25-tetraol (M2) in rat plasma. 20-( S)-Protopanaxatriol, M1, and M2 were extracted with methanol and separated on an ACQUITY HSS T3 column. The mass spectrometry detection was accomplished in selected reaction monitoring mode with precursor-to-product ion transitions of m/z 493.4→143.1 for M1 and M2, m/z 475.4→391.3 for 20-( S)-protopanaxatriol, and m/z 459.4→375.3 for 20-( S)-protopanaxadiol (internal standard). The method showed good linearity over the concentration ranges of 1-1000 ng/mL for 20-( S)-protopanaxatriol and 0.5-200 ng/mL for M1 and M2, with correlation coefficients of more than 0.995. The lower limits of quantification for 20-( S)-protopanaxatriol, M1, and M2 were 1, 0.5, 0.5 ng/mL, respectively. The intra- and interday precisions (RSD, %) were less than 10.41% while the accuracy (relative error, %) ranged from -3.14 to 8.73%. Under the current conditions, M1 and M2 were completely separated within 3 min. The validated assay was successfully applied to evaluating pharmacokinetic profiles of 20-( S)-protopanaxatriol, M1, and M2 in rat. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16159306
- Volume :
- 40
- Issue :
- 23
- Database :
- Academic Search Index
- Journal :
- Journal of Separation Science
- Publication Type :
- Academic Journal
- Accession number :
- 126587248
- Full Text :
- https://doi.org/10.1002/jssc.201700909