Back to Search
Start Over
Defense response of Eucalyptus camaldulensis against black spot pathogen of Pisum sativum.
- Source :
-
South African Journal of Botany . Nov2017, Vol. 113, p428-436. 9p. - Publication Year :
- 2017
-
Abstract
- Ascochyta pisi induces leaf spot in Pisum sativum . Currently A . pisi was found pathogenic against 4 pea varieties. Ten concentrations of Eucalyptus camaldulensis leaf extract and its 7 fractionations were appraised for antifungal activity against A . pisi . The highest concentration revealed 78% suppression in growth. Fractionation was carried out successively with n-hexane, Chloroform, Ethyl acetate and n-Butanol. In in vivo bioassays, all the solvent fractions particularly n-Butanol controlled pathogen efficiently on all varieties. In RT-PCR, all genes were expressed in control and treated plants of Greenfeast but the intensity of gene 2 and 8 was upregulated in treated plants. n-Butanol induced the over expression of gene 3 and 7 in Meteor. Then, in native PAGE Glu 1 was the glucanase isozyme, detected only in variety Greenfeast. Control plants of Rondo showed Glu 2 and 3, while its treated plants had Glu 1, Glu 2, Glu 3 and Glu 4. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02546299
- Volume :
- 113
- Database :
- Academic Search Index
- Journal :
- South African Journal of Botany
- Publication Type :
- Academic Journal
- Accession number :
- 126438613
- Full Text :
- https://doi.org/10.1016/j.sajb.2017.09.021