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Spectrophotometric and Electrochemical Determination of MicroRNA-155 Using Sandwich Hybridization Magnetic Beads.
- Source :
-
Analytical Letters . 2018, Vol. 51 Issue 3, p411-423. 13p. - Publication Year :
- 2018
-
Abstract
- Here a new colorimetric assay and electrochemical biosensor for microRNA detection based on magnetic bead separation and enzyme amplification are reported. MicroRNA-155 was selected as a model because of its involvement in breast and lung cancer. A novel platform was developed for label-free microRNA detection based on a sandwich assembly using streptavidin magnetic beads coupled with a biotinylated capture probe that hybridized only a portion of the microRNA, the remainder hybridized with biotinylated detection probe linked to streptavidin-alkaline phosphatase. After appropriate reaction with the substrate, the enzymatic product was evaluated with colorimetric and electrochemical detection. The electrochemical method showed high sensitivity, allowing microRNA detection at 29 pmol L−1, which correspond to 1.4 fmole in 50 μL with a linear range from 81 pmol L−1to 1.2 nmol L−1. This simple and efficient biosensor may have application in clinical molecular diagnostics and biomedical research. [ABSTRACT FROM PUBLISHER]
Details
- Language :
- English
- ISSN :
- 00032719
- Volume :
- 51
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Analytical Letters
- Publication Type :
- Academic Journal
- Accession number :
- 126433598
- Full Text :
- https://doi.org/10.1080/00032719.2017.1302460