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In-Situ Self-Assembly of Zinc/Adenine Hybrid Nanomaterials for Enzyme Immobilization.
- Source :
-
Catalysts (2073-4344) . Nov2017, Vol. 7 Issue 11, p327. 14p. - Publication Year :
- 2017
-
Abstract
- In this study, a one-step and facile immobilization of enzymes by self-assembly of zinc ions and adenine in aqueous solution with mild conditions was reported. Enzymes, such as glucose oxidase (GOx) and horseradish peroxidase (HRP), could be efficiently encapsulated in Zn/adenine coordination polymers (CPs) with high loading capacity over 90%. When the enzyme was immobilized by CPs, it displayed high catalytic efficiency, high selectivity and enhanced stability due to the protecting effect of the rigid framework. As a result, the relative activity of Zn/adenine nano-CP-immobilized GOx increased by 1.5-fold at pH 3 and 4-fold at 70 to 90 ˚C, compared to free GOx. The immobilized GOx had excellent reusability (more than 90% relative activity after being reused eight times). Furthermore, the use of this system as a glucose biosensor was also demonstrated by co-immobilization of two enzymes, detecting glucose down to 1.84 µM with excellent selectivity. The above work indicated that in-situ self-assembly of Zn/adenine CPs could be a simple and efficient method for biocatalyst immobilization. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GLUCOSE oxidase
*HORSERADISH peroxidase
*COORDINATION polymers
Subjects
Details
- Language :
- English
- ISSN :
- 20734344
- Volume :
- 7
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Catalysts (2073-4344)
- Publication Type :
- Academic Journal
- Accession number :
- 126429674
- Full Text :
- https://doi.org/10.3390/catal7110327