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Over-expression of anti-CD75 reactive proteins on distal and collecting renal tubular epithelial cells in calcium-oxalate stone-forming kidneys in Egypt.
- Source :
-
BJU International . Apr2004, Vol. 93 Issue 6, p822-826. 5p. - Publication Year :
- 2004
-
Abstract
- To assess the nature, distribution and expression pattern of CD75, a neuraminidase-sensitive lymphocyte cell surface differentiation antigen, in calcium oxalate (CaOx) stone disease, as cell-surface sialic acid might be involved CaOx crystal binding, and lectin-binding assays suggest that sialic acid in the α2,6 position is upregulated in stone-forming kidneys. Human CaOx stone-forming and normal kidneys (13 each) and primary kidney epithelial cells (CAKI-1, three samples) were analysed. The protein pattern, distribution and expression of CD75 were analysed using Western blotting, immunohistology and semi-quantitative confocal laser scanning microscopy (cLSM). Production was investigated by α2,6-sialyltransferase specific reverse transcription-polymerase chain reaction. Western blotting showed one strong band at ≈ 43 kDa that reacted with anti-CD75 when renal epithelial and CAKI-1 tumour cell extracts were analysed. However, in renal tissue extracts of CaOx stone formers there were additional bands at 120 and 205 kDa. Image processing after cLSM showed that anti-CD75 reactivity was significantly greater on E-cadherin-positive distal and collecting tubular cells from CaOx stone-forming kidneys, at a mean ( sd) intensity of 87 (7), than on those from normal kidneys, at 41 (5) ( P = 0.005). CD75 expression in human kidney was primarily on the luminal surface of distal tubules and collecting ducts. Whether increased epithelial CD75 expression in CaOx stone disease is a cause or result of the disease remains to be clarified. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14644096
- Volume :
- 93
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- BJU International
- Publication Type :
- Academic Journal
- Accession number :
- 12628109
- Full Text :
- https://doi.org/10.1111/j.1464-410x.2003.04751.x