BIOMECHANICAL PROPERTIES OF DELIPIDIZED STRATUM CORNEUM.

Alterations of the physical properties of stratum corneum induced by ether extraction were studied. In vitro changes measured included mechanical work to stretch (Instron extensometer), cross-sectional swelling, and expansion in the plane of the tissue when placed in water. Results showed that ether extraction produced a pronounced weakening of the stratum corneum as shown by the work index when measured at 5% extension. This weakening was accompanied by an increase in the cross-sectional swelling and higher expansion in the plane of the tissue. In vivo observations using scanning electron microscopy of replicas of ether-extracted skin confirmed the pronounced swelling effects seen in vitro. The results suggest that removal of lipids from the surface of the skin produces a pronounced weakening and swelling when the skin is subsequently immersed in water. This may be reflected in the appearance of cracks and upturned stratum corneum cells. [ABSTRACT FROM AUTHOR]