1840 MHz 射频辐射对大鼠血脑屏障通透性的影响.

Objective: With the development of modern communication and the popularization of mobile phone, the exposure level of radiofrequency (RF) radiation emitted from mobile phone has been increased unprecedentedly, which leads to extensive public concern about the influences of RF radiation on human health. This investigation was designed to explore the effects of environmental radio frequency fields on the permeability of blood brain barrier (BBB). Methods: 36 healthy adult male SD rats were assigned to sham group and exposure group randomly. The rats of exposure group were exposed to 1840 MHz at specific absorption rate (SAR) of 2 W/kg for 1 h/day and 7 days totally. After RF exposure, the rats were sacrificed immediately. Transmission electron microscope (TEM) of exogenous lanthanum nitrate (La3+)-tracing, albumin immunohistochemical staining and western blotting were performed to observe the permeability of BBB and the protein level of tight junction (TJ) associated protein zonula occludens-1 (ZO-1). Results: The cerebral cortex of sham group showed general normal appearance of nerve cells and endothelial cells. The lanthanum nitrate was confined to the lumen. However in exposure group, there was linear deposition of lanthanum particles between the endothelial cells and the basement membrane as well as in the brain parenchyma. Similarly, albumin immunoactivity was found around cortical microvessels. These results indicated that the permeability of BBB increased after RF exposure. Meanwhile, western blot results showed that the expression level of ZO-1 decreased slightly (t=5.438, P < 0.001) compared with the sham group. At the level of α=0.05, the difference was statistically significant. Conclusions: Exposure to radio frequency fields at 1840 MHz could increase the BBB permeability, and probably tight junction associated protein ZO-1 was involved in it. In this context, people who have been exposed to RF radiation with high SAR value for long time may suffer fromhigh risk of BBB breakdown and CNS disorder. [ABSTRACT FROM AUTHOR]