Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a:K30.

A novel integral membrane lipoprotein, Wza, from Escherichia coli serotype O9a:K30 has been purified and crystallized. Wza is required for the surface expression of the serotype K30 group 1 capsular polysaccharide of E. coli; closely related homologues are found in other bacteria that produce extracellular polysaccharides. The Wza crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 94.6, b = 215.5, c = 218.5 Å. A data set to 3.0 Å with 99.8% completeness and an Rmerge of 10.5% has been collected from a single crystal. [ABSTRACT FROM AUTHOR]