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Studies on Phenylalanine-Specific Transfer Ribonucleic Acid from Chick Embryos.
- Source :
-
European Journal of Biochemistry . Dec74 Part 1, Vol. 49 Issue 3, p521-529. 9p. - Publication Year :
- 1974
-
Abstract
- The phenylalanine-specific tRNAPhe from 13-day-old chick embryo muscle was purified. Its fluorescent and hydrophobic Y+ base was excised by mild acid treatment and characterized chromatographically and by its ultraviolet absorption and fluorescent spectra. The compound obtained after removal of the Y+ base from chick embryo tRNAPhe was characterized. 1. Chick embryo t RNAPhe looses by the removal of the Y+ base its phenylalanine acceptor capacity. 2. In this state it has a reduced sedimentation coefficient of 2.8 S. a low Tm value (22 °C), only rudimentary hyperchromicity, but unchanged electrophoretic properties. 3. The compound obtained after removal of the Y+ base from chick embryo tRNAPhe regains normal melting behaviour and phenylalanine acceptor capacity when exposed to special renaturation conditions. 4. The Km of the renatured form is 0.25 × 10-6 M and V = 220 pmol × min-1 × ml-1 as compared tO Km = 0.11 × 10-6 M and V = 370 pmol x min-1 × ml-1 for the native chick embryo tRNAPhe. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 49
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12596497
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1974.tb03856.x