ANTIMICROBIAL SCREENING AND SYNTHESIS OF QUINAZOLINONE DERIVATIVES.

The present work deals with synthesis of 2-Pyridyl - 4 Quinazolone (NN) (A1), 2-Pyrazinyl - 4 - Quinazolone (A2).The synthesis is based on Niementowski Reaction which involves condensation of the reactants under varying thermal conditions. The synthesis of 2-hetero substituted Quinazolone is based on Niementowski Reaction involves thermal condensation and cyclisation of a drug amide and Anthranilic acid / 3, 5 di bromo anthranilic acids at elevated temperatures to yield the various hetero-moieties. The drug amides used are Nicotinamide, Pyrazinamide and Carbamezepine. The products formed were recrystallized using ethanol. Thin layer chromatography was carried out using Benzene: ethanol. The melting points of the products were found by capillary tube method. The values are uncorrected. The synthesized compounds have been characterized using UV, IR, NMR and MASS. The literature review indicates that Substituted Quinazolinones have wide spectrum of pharmacological activity such as anti-bacterial, antimicrobial, antiviral and antifungal. Hence antimicrobial study was carried out. The antibacterial activity of the synthesized compounds was determined .The pathogens used in this study included both gram positive and gram negative organisms. Namely Negative coagulase staphylococci, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, all the synthesized compounds showed antibacterial activity to varying degree against the organisms tested. Zone of inhibition was carried out using Antibiotic disc diffusion assay method Amikacin (5μg/ml) was used as control. [ABSTRACT FROM AUTHOR]