Back to Search
Start Over
In vitro transdifferentiation of adult pancreatic acinar cells into insulin-expressing cells
- Source :
-
Biochemical & Biophysical Research Communications . Apr2004, Vol. 316 Issue 4, p1094. 7p. - Publication Year :
- 2004
-
Abstract
- Despite a recent breakthrough in human islet transplantation for treating diabetes mellitus, the limited availability of insulin-producing tissue is still a major obstacle. Here, we studied whether adult pancreatic acinar cells have the potential to transdifferentiate into islet or β cells. Pancreatic acini were isolated from 7- to 8-weeks-old male Sprague–Dawley rats and cultured in suspension. Within 1 week, most of the acinar cells lost amylase expression and converted to cells with a duct cell phenotype. Insulin-positive cells were also observed, mainly at the periphery of the acini-derived spheroids. Insulin gene and protein expression was increased. Presence of a few insulin-positive cells coexpressing cytokeratins suggests that a spontaneous acinar to ductal cell transdifferentiation process was further going on towards β cells. This study provides the first evidence that adult pancreatic acinar cells could be differentiated into insulin-expressing cells in vitro. [Copyright &y& Elsevier]
- Subjects :
- *ISLANDS of Langerhans
*DIABETES
*TISSUES
*CELLS
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 316
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 12576985
- Full Text :
- https://doi.org/10.1016/j.bbrc.2004.02.153