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Enhanced cytochrome P450 capacities of Caco-2 and Hep G2 cells in new coculture system under the static and perfused conditions: evidence for possible organ-to-organ interactions against exogenous stimuli
- Source :
-
Materials Science & Engineering: C . Apr2004, Vol. 24 Issue 3, p333. 7p. - Publication Year :
- 2004
-
Abstract
- To investigate particularly organ-to-organ interactions against the exogenous stimuli, two coculture systems, i.e. a simple double-layered static coculture system and a perfusion coculture system consisting of Caco-2 and Hep G2 cells to mimic the metabolic processes occurring in the small intestine and the liver, were used. The induction of cytochrome P450 (CYP) 1A1/2 was measured to estimate the metabolic capacity of Caco-2 and Hep G2 cells against tested chemicals.In both coculture systems, the inducibility of CYP1A1/2 by benzo[a]pyrene (B[a]P) or 3-methylcholanthrene (3-MC) in terms of ethoxyresorufin O-dimethylase (EROD) activity was more remarkable than expected from the individual cell cultures. It seemed to show possible organ-to-organ interactions in terms of the physiological protection of human body against exogenous stimuli. Therefore, we can say that a real-time combined system is really important to predict the complicated phenomena in human because an individual culture system cannot represent the organ-to-organ interaction and also coculture systems can give information of final metabolic activity as a result of many complicated phenomena. [Copyright &y& Elsevier]
- Subjects :
- *CYTOCHROMES
*CELLS
*CYTOCHROME P-450 CYP1A1
*METABOLISM
Subjects
Details
- Language :
- English
- ISSN :
- 09284931
- Volume :
- 24
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Materials Science & Engineering: C
- Publication Type :
- Academic Journal
- Accession number :
- 12575859
- Full Text :
- https://doi.org/10.1016/j.msec.2003.12.002