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Characterization of an ionic liquid-tolerant β-xylosidase from a marine-derived fungal endophyte.
- Source :
-
Biochemistry & Cell Biology . 2017, Vol. 95 Issue 5, p585-591. 7p. - Publication Year :
- 2017
-
Abstract
- Ionic liquids (ILs) are used in lignocellulosic biomass (LCB) pretreatment because of their ability to disrupt the extensive hydrogen-bonding network in cellulose and hemicellulose, and thereby decrease LCB recalcitrance to subsequent enzymatic degradation. However, this approach necessitates the development of cellulases and hemicellulases that can tolerate ∼20% ( w/ v) IL, an amount that either co-precipitates with the sugar polymers after the initial pretreatment or is typically used in single-pot biomass deconstructions. By investigating the secretomes from 4 marine-derived fungal endophytes, we identified a β-xylosidase derived from Trichoderma harzianum as the most promising in terms of tolerating 1-ethyl-3-methylimidazolium-dimethyl phosphate (EMIM-DMP), an IL. When tested with p-nitrophenyl-β- d-xyloside, this extracellular xylosidase retained ∼50% activity even in 1.2 mol·L-1 (20% w/ v) EMIM-DMP after incubation for 48 h. When tested on the natural substrate xylobiose, there was ∼85% of the initial activity in 1.2 mol·L-1 EMIM-DMP after incubation for 9 h and ∼80% after incubation for 48 h. Despite previous findings associating thermostability and IL tolerance, our findings related to the mesophilic T. harzianum β-xylosidase(s) emphasize the need to include the marine habitat in the bioprospecting dragnet for identification of new IL-tolerant LCB-degrading enzymes. [ABSTRACT FROM AUTHOR]
- Subjects :
- *IONIC liquids
*XYLOSIDASES
*ENDOPHYTIC fungi
*HEMICELLULOSE
*ENDOPHYTES
Subjects
Details
- Language :
- English
- ISSN :
- 08298211
- Volume :
- 95
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Biochemistry & Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- 125460328
- Full Text :
- https://doi.org/10.1139/bcb-2017-0053