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The effect of allergen-induced bronchoconstriction on concentration of 5-oxo-ETE in exhaled breath condensate of house dust mite-allergic patients.
- Source :
-
Clinical & Experimental Allergy . Oct2017, Vol. 47 Issue 10, p1253-1262. 10p. - Publication Year :
- 2017
-
Abstract
- Background Arachidonic acid metabolites regulate several aspects of airway function including inflammation, muscle contraction and mucous secretion. Objective The aim of this study was to evaluate concentration of selected 5-lipoxygenase- and cyclooxygenase-derived eicosanoids in exhaled breath condensate (EBC) during allergen-induced bronchoconstriction. Methods The study was performed on 24 allergic rhinitis/asthma patients sensitized to a house dust mite (HDM) Dermatophagoides pteronyssinus (Dp) and 13 healthy controls (HCs). Bronchial challenge with Dp extract was performed only in the allergic patients. EBC samples were collected before ( T0) and during Dp-induced bronchoconstriction ( TEAR). Eicosanoid concentration was measured using HPLC-tandem mass spectrometry. Results Significant bronchoconstriction after Dp challenge was demonstrated in 15 patients (Rs), while in 9 patients (NRs) no asthmatic response could be detected. At T0 the most abundant eicosanoids in EBC of HDM-allergic patients were LTB4 and 5-oxo-ETE, while in HCs EBC concentration of LTB4 was significantly greater than that of 5-oxo-ETE. Allergen challenge resulted in significant increase in EBC concentration of 5-oxo-ETE, LTD4 and 8-iso-PGE2 only in Rs. At TEAR, the relative change of 5-oxo-ETE concentration in EBC correlated with decrease of peripheral blood eosinophilia ( R = −0.774; P = .0012). Moreover, the relative increase of 5-oxo-ETE in EBC at TEAR significantly correlated with the severity of the subsequent late asthmatic response ( R = 0.683, P = .007). Conclusion Our study demonstrates significant up-regulation of 5-oxo-ETE synthesis in HDM-allergic patients and indicates possible involvement of that mediator in the pathogenesis of allergic asthma. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ARACHIDONIC acid
*METABOLITES
*INFLAMMATION
*CYCLOOXYGENASES
*EICOSANOIDS
Subjects
Details
- Language :
- English
- ISSN :
- 09547894
- Volume :
- 47
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Clinical & Experimental Allergy
- Publication Type :
- Academic Journal
- Accession number :
- 125381578
- Full Text :
- https://doi.org/10.1111/cea.12990