Back to Search
Start Over
Determination of molar masses of macromolecules by size exclusion chromatography-light scattering not requiring knowledge of refractive index increments.
- Source :
-
Journal of Chromatography A . Oct2017, Vol. 1520, p103-106. 4p. - Publication Year :
- 2017
-
Abstract
- A new approach for the calibration of SEC-light scattering (SEC-LS) setups is proposed, which requires solely the molar mass of a reference polymer. Neither the specific refractive index increment of the calibrant nor of the analyte is required. Comparison of the molar masses derived in different solvents for a large number of chemically different polymers shows that the new approach yields the same molar masses as if molar masses were derived using dn/dc to calibrate the light scattering setup. The approach therefore allows easier determination of molar masses by SEC-LS. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219673
- Volume :
- 1520
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography A
- Publication Type :
- Academic Journal
- Accession number :
- 125358826
- Full Text :
- https://doi.org/10.1016/j.chroma.2017.09.011