Back to Search
Start Over
Molecular characterization and antimicrobial resistance profile of Clostridium perfringens type A isolates from humans, animals, fish and their environment.
- Source :
-
Anaerobe . Oct2017, Vol. 47, p120-124. 5p. - Publication Year :
- 2017
-
Abstract
- The study was aimed to characterize, and determine antibiogram of C. perfringens type A isolated from the feces of human and animal diarrhoeal cases, as well as healthy animals, meat of pigs and goats, gills and intestine of fish and samples from fish pond. A total of 460 samples, including human diarrhoeal cases (n = 130); diarrhoeal cases of pig (n = 52) and goat (n = 50); fecal samples from healthy pig (n = 50) and goat (n = 50); meat samples viz . pork meat (n = 52); goat meat (n = 50) and fish including their environmental sources (n = 26) were used for isolation and identification of C. perfringens type A. All the biochemically confirmed isolates were positive for species-specific 16S rRNA and cpa genes by PCR assays. Toxinotyping of C. perfringens type A isolates showed that overall prevalence of C. perfringens type A with only cpa + gene was 43.2%; with cpa + and cpb2 + genes was 45.4%; with cpa + and cpe + genes was 4.9%; however, with cpa + , cpb2 + and cpe + genes was 6.6%. Antimicrobial susceptibility testing revealed that 83.7% of isolates were resistant to three or more antibiotics. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10759964
- Volume :
- 47
- Database :
- Academic Search Index
- Journal :
- Anaerobe
- Publication Type :
- Academic Journal
- Accession number :
- 125338242
- Full Text :
- https://doi.org/10.1016/j.anaerobe.2017.05.009