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Block V RTX Domain of Adenylate Cyclase from Bordetella pertussis: A Conformationally Dynamic Scaffold for Protein Engineering Applications.
- Source :
-
Toxins . Sep2017, Vol. 9 Issue 9, p289. 13p. - Publication Year :
- 2017
-
Abstract
- The isolated Block V repeats-in-toxin (RTX) peptide domain of adenylate cyclase (CyaA) from Bordetella pertussis reversibly folds into a β-roll secondary structure upon calcium binding. In this review, we discuss how the conformationally dynamic nature of the peptide is being engineered and employed as a switching mechanism to mediate different protein functions and protein-protein interactions. The peptide has been used as a scaffold for diverse applications including: a precipitation tag for bioseparations, a cross-linking domain for protein hydrogel formation and as an alternative scaffold for biomolecular recognition applications. Proteins and peptides such as the RTX domains that exhibit natural stimulus-responsive behavior are valuable building blocks for emerging synthetic biology applications. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20726651
- Volume :
- 9
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Toxins
- Publication Type :
- Academic Journal
- Accession number :
- 125283620
- Full Text :
- https://doi.org/10.3390/toxins9090289