丹红注射液中5种主要活性成分促血管新生作用研究.

Objective: To explore the proangiogenic activities of five main components from Danhong injection (DHI) absorbed in blood. Metiiods: A in vitro culture system of human umbilical vein endothelial cells (HUVEC) and a model ofchicken embryo chorioallantoic membrane (CAM) were used in this study. Cells or chicken embryos were randomly divided into control (Con) group, DHI group, hydroxysafflor yellow A (HSYA) group, salvianolic acid B (Sal B) group, Tanshinol (Tan) group, protocatechuic aldehyde (Pra) group and Rosmarinic acid (RosA) group. Results: In comparison with Con group, 10% (V /V )DHI and its five main components at corresponding concentrations could significantly promote HUVEC proliferation (P<0.01) and the expression of vascular endothelial growth factor A (VEGF-A), basic fibroblast growth Factor (bFGF) and hepatocyte growth factor (HGF) in HUVEC (P<0.05, P<0.01). Also, 50 % (V /V )DHI and these components at corresponding concentrations were able to boost the blood vessel density on CAM to different extent. Consistently, HSYA and Tan showed better proangiogenic action in both models. Conclusions: All the five main components from Danhong injection (DHI) absorbed in blood promote angiogenesis and HSYA and Tan have stronger proangiogenic activities than the others. [ABSTRACT FROM AUTHOR]